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Image Search Results
Journal: bioRxiv
Article Title: The connectome is necessary but not sufficient for the spread of alpha -synuclein pathology in rats
doi: 10.1101/567222
Figure Lengend Snippet: (a) In each published mouse connectivity experiment from Allen Brain Institute (Oh et al. 2014), an injection of an anterograde tracer, recombinant adeno-associated virus (rAAV) expressing enhanced green fluorescent protein (GFP, green arrow), results in a focus of cerebral fluorescence (shaded green), that is maximal in a primary source structure (XII). However, adjacent structures may also fluoresce, two of which, with the most pixels fluorescing, relative to their volume, we designate as secondary source structures (DMX, MDRN). Our composite mouse mesoscale connectome was reconstructed from 207 independent connectivity experiments, with projections emanating from one primary and two secondary structures per experiment (three source structures). (b) In the published rat spreading experiment , the left vagus nerve (yellow) is injected with adeno-associated virus, expressing human alpha -synuclein, at a position (red arrow) distal to the inferior ganglion of the vagus (IGX). This primarily infects nerve fascicles and tracts (red curves) and neuronal soma (red ovals) of brain structures (DMX, AMB) and ganglionic neurons (IGX) located in the vagal trunk, outside of the central nervous system. Thereby, axons within brain structures that receive vagal projections (NTS, AP, DMX) may also express human alpha -synuclein. However ganglionic neurons and their central projections that branch out from the vagal trunk at positions proximal to the injection site, are not directly infected, e.g. the superior ganglion of the vagus (SGX) and the corresponding caudal part of the spinal nucleus of the trigeminal nerve (SPVC).
Article Snippet:
Techniques: Injection, Recombinant, Expressing, Fluorescence, Infection